-
祝贺2023届硕士毕业生顺利通过答辩!
-
欢送 2022届硕士毕业生!
恭喜2022届硕士毕业生在上海严峻疫情的大背景下顺利毕业!
-
Static Magnetic Field Inhibits Growth of Escherichia coli Colonies via Restriction of Carbon Source Utilization
Magnetobiological effects on growth and virulence have been widely reported in Escherichia coli (E. coli). However, published results are quite varied and sometimes conflicting because the underlying mechanism remains unknown. Here, we reported that the application of 250 mT static magnetic field (SMF) significantly reduces the diameter of E. coli colony-forming units (CFUs) but has no impact on the number of CFUs.
-
欢迎来到黄lab!
实验室网站正式上线!
-
The pentatricopeptide repeat protein EMB1270 interacts with CFM2 to splice specific group II introns in Arabidopsis chloroplasts
Chloroplast biogenesis requires the coordinated expression of chloroplast and nuclear genes. Here, we show that EMB1270, a plastid-localized pentatricopeptide repeat (PPR) protein, is required for chloroplast biogenesis in Arabidopsis thaliana. Knockout of EMB1270 led to embryo arrest, whereas a mild knockdown mutant of EMB1270 displayed a virescent phenotype. Almost no photosynthetic proteins accumulated in the albino emb1270 knockout mutant. By contrast, in the emb1270 knockdown mutant, the levels of ClpP1 and photosystem I (PSI) subunits were significantly reduced, whereas the levels of photosystem II (PSII) subunits were normal. Furthermore, the splicing efficiencies of the clpP1.2, ycf3.1, ndhA, and ndhB plastid introns were dramatically reduced in both emb1270 mutants. RNA immunoprecipitation revealed that EMB1270 associated with these introns in vivo. In an RNA electrophoretic mobility shift assay (REMSA), a truncated EMB1270 protein containing the 11 N-terminal PPR motifs bound to the predicted sequences of the clpP1.2, ycf3.1, and ndhA introns. In addition, EMB1270 specifically interacted with CRM Family Member 2 (CFM2). Given that CFM2 is known to be required for splicing the same plastid RNAs, our results suggest that EMB1270 associates with CFM2 to facilitate the splicing of specific group II introns in Arabidopsis.
-
Lift the mystery veil on chlorophyllases
Photosystem II (PSII) is a large membrane-protein complex that catalyzes the splitting of water into protons, electrons, and oxygen via harvesting light energy (Johnson, 2016). The reaction center of PSII, operating like an electron pump, is a special pair of chlorophyll a (Chl a), referred to as P680, bound to D1 and D2 subunits, each with its set of electron carriers. Upon absorption of light energy, P680 is excited and donates an electron to the neighboring pheophytin (Pheo), producing a pair of radicals (⋅P680+ and ⋅Pheo−), called charge separation.
-
欢送 2020届硕士毕业生!
恭喜2020届硕士毕业生在席卷全球的疫情大背景下顺利毕业!